DESCRIPTION :
This research focuses on deep-sea marine Archaea from the order Thermococcales, isolated from hydrothermal vent ecosystems. These microorganisms thrive under extreme conditions such as high temperature, pressure, and variable nutrient availability. As such, they offer relevant models to investigate stress responses, energy regulation, and the evolution of molecular adaptation strategies.
What will your mission BE?
The recruited PhD student will contribute to advancing our understanding of how Archaea-microorganisms thriving in extreme environments-adapt their metabolism and protein synthesis machinery under stress conditions. By investigating the role of a newly identified ribosome hibernation factor (HIB) and its interaction with a key metabolic enzyme, this thesis aims to uncover fundamental mechanisms of biological regulation that remain largely unexplored. The project lies at the crossroads of microbiology, biochemistry, and systems biology, and seeks to shed light on cellular plasticity, stress adaptation, and the evolution of survival strategies across life forms.
What will your activities BE?
This PhD project, hosted at the Ifremer BEEP laboratory, will focus on :
- The phenotypic characterization of the hib mutant compared to wild-type strains under physiological and stress conditions (Growth kinetics, viability, luciferase assays, Western blotting).
- Comparative transcriptomic and proteomic analysis of wild-type, hib, and mutants strains deleted from targeted metabolic enzymes to identify affected pathways.
- In vitro enzymatic assays to characterize the impact of HIB, on key metabolic enzymes, under varying energy charge (ATP/ADP/AMP).
How are your activities organised?
- 100%, teleworking possible, Phd is a real opportunity to work on Ifremer's scientific and technological priority themes. IT entitle the holder to a gross monthly salary of 2300 Euros gross for a period of 3 years, which cannot BE combined with other scholarships.
- We have a teleworking agreement and our staff have 12 comp time days and supra statutory leave.
- We allocate a significant budget to developing skills, through individual or collective training actions.
- We have a CSR policy inspired by the purposes of the institute (sustainable mobility allowance, etc.), Doctoral students' contracts will start last quarter of 2025, subject to the submission of administrative documents authorizing Ifremer to recruit the doctoral student (certificate of completion of the Master 2 or engineering degree + visa for foreign doctoral students outside the EU).
Code d'emploi : Chercheur en Pharmacie (h/f)
Domaine professionnel actuel : Experts Chimistes et Laborantins
Niveau de formation : Bac+3
Temps partiel / Temps plein : Plein temps
Type de contrat : Franchise
Compétences : Microsoft Excel, Analyse des Données, R (Langage de Programmation), Python (Langage de Programmation), Automatic Data Processing, Gestion du Stress, Curiosité, Biochimie, Biologie Molleculaire, Biologie, Métabolisme, Transcription (Génétique), Enzymes, Enzymologie, Western Blot, Conduite de Machine, Métabolomique, Microbiologie, Gestion Administrative, Biologie des Systèmes, Protéomique
Courriel :
dirbrest@ifremer.fr
Téléphone :
0298224040
Type d'annonceur : Employeur direct